Autoclaves are essential tools in microbiology for sterilizing equipment and materials.
They ensure that all microorganisms, including spores, are killed, making them safe for use in laboratories.
Here are the different types of autoclaves commonly used in microbiology:
1. Pressure Cooker Type/Laboratory Bench Autoclaves (N-type)
These autoclaves are widely used in laboratories around the world.
They feature an air and steam discharge tap, a safety valve, and a pressure gauge.
An electric immersion heater is located at the bottom of the chamber.
2. Gravity Displacement Autoclaves
These autoclaves are commonly found in microbiology and medical college labs.
They generate steam inside the chamber through a heating unit that can move within the chamber for optimal sterilization.
They are relatively cheaper compared to other types of autoclaves.
3. Positive Pressure Displacement Autoclaves (B-type)
These autoclaves use positive pressure to displace the air inside the chamber, ensuring thorough sterilization.
They are commonly used in medical and dental settings.
4. Negative Pressure Displacement Autoclaves (S-type)
These autoclaves use negative pressure to remove air from the chamber before sterilization.
They are commonly used in research laboratories and pharmaceutical industries.
5. Vacuum Pressure Autoclaves
These autoclaves use vacuum pressure to remove air from the chamber, creating a low-pressure environment for sterilization.
They are known for their efficiency and are commonly used in industries where high-quality sterilization is required.
It's important to note that while there are different types of autoclaves, they all operate in a similar manner.
The type of autoclave chosen depends on the setting in which it will be used.
Autoclaves are trusted sterilization devices in microbiology because they are capable of killing microorganisms that are resistant to boiling water and other powerful detergents.
They are recognized as the most powerful sterilization method available to microbiologists.
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