When it comes to sterilization in an incubator, specifically an autoclave, the recommended temperature range is between 121°C (250°F) and 135°C (275°F).
This high temperature is crucial for effectively killing microorganisms on the items being sterilized.
4 Key Points to Consider
1. Temperature Settings
The commonly recommended temperatures for steam sterilization are 121°C (250°F), 132°C (270°F), or 135°C (275°F).
These temperatures are selected because they are high enough to destroy a wide range of microorganisms, including bacteria, viruses, fungi, and spores.
2. Process of Sterilization
The sterilization process in an autoclave involves boiling water to generate steam, which then enters the chamber.
The air in the chamber is removed either by a vacuum pump or by displacement, depending on the size of the autoclave.
Once all the air is removed, the exhaust valve is closed to increase pressure and temperature.
The steam, which is highly pressurized, allows its temperature to exceed 100°C, making it effective for sterilization.
3. Time and Temperature Relationship
The time required for sterilization depends on the temperature and pressure set in the autoclave.
For example, at 121°C and a pressure of 108 kilopascals (kPa), sterilization typically takes 20 minutes.
However, at a higher temperature of 134°C and a pressure of 206 kPa, sterilization can be achieved in just 10 minutes.
This inverse relationship between pressure/temperature and time highlights the efficiency of higher temperatures in reducing the sterilization time.
4. F0 Value
Some advanced autoclaves use an F0 value to standardize the sterilization process.
The F0 value calculates the equivalent sterilization time at 121°C.
This helps in ensuring consistent sterilization across different cycles and conditions, adjusting for variations in temperature and ensuring that the sterilization process meets the required Sterility Assurance Level.
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