Autoclave maintenance should be performed on a routine basis, with specific frequencies depending on the type of testing and the regulatory requirements of the facility. Routine preventative maintenance inspections should be conducted monthly, quarterly, or annually to ensure the autoclave remains in proper working order. These inspections cover components such as heating coils, contactors, steam traps, and safety valves.
For monitoring and testing protocols, autoclaves must be tested before being placed into service and routinely afterward. In Florida, for instance, autoclaves used to inactivate substances like human pathogens, blood, tissues, and clinical samples must be tested after every 40 hours of use. Autoclaves used to sterilize other materials must be tested every six months. Additionally, biological indicators (BIs) should be used at least once per month to verify the adequacy of the decontamination process. In higher-risk laboratories or when loads show significant variation, BI monitoring may be required more frequently, potentially even in every load. The methods and frequency of BI monitoring should be evaluated based on microbiological risk assessments.
In summary, the frequency of autoclave maintenance and testing varies based on the specific use of the autoclave and local regulations. Regular inspections and tests are crucial to ensure the autoclave's effectiveness and to prevent system downtime and costly repairs.
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