When using an autoclave, it's crucial to avoid certain practices to ensure safe and effective sterilization. Here are 12 key points to keep in mind:
12 Key Things to Avoid When Using an Autoclave
1. Do not autoclave cracked or compromised glassware
Cracks in glassware can compromise their integrity and lead to breakage during the autoclave process. Always inspect glassware for any cracks and discard them if found.
2. Do not autoclave waterproof or dry materials
Autoclaves use steam to sterilize. Materials that are waterproof or dry, such as oil, grease, or glove powder, should not be autoclaved as they may not be effectively sterilized.
3. Ensure efficient steam penetration
Proper loading of materials is crucial for effective sterilization. Overfilling the autoclave chamber can hinder steam penetration. It's recommended to run two separate, uncrowded loads instead of one crowded load. Materials should be loaded in a way that allows for efficient steam circulation and should not touch the sides or top of the chamber.
4. Avoid wrapping objects in aluminum foil
Wrapping objects in aluminum foil can interfere with steam penetration and may lead to inadequate sterilization. Instead, use materials that allow steam to penetrate.
5. Autoclave clean items and wastes separately
It's important to autoclave clean items and wastes separately to prevent contamination of the clean items.
6. Avoid using polyethylene trays
Polyethylene trays should not be used in the autoclave as they can melt and cause damage to the autoclave.
7. Wear suitable Personal Protective Equipment (PPE)
When operating an autoclave, it's important to wear appropriate PPE, such as a lab coat, flame-resistant gloves, and protective eyewear, to protect against potential hazards.
8. Do not open the door if water is coming out from the bottom
If water starts coming out from the bottom of the autoclave, do not open the door. This can be caused by obstructed steam lines, equipment breakdown, or clogged drains. Contact the appropriate personnel for assistance.
9. Avoid contact between material and autoclave walls
While loading the autoclave, ensure that the material does not touch the internal walls of the autoclave. This can help to prevent uneven heating and potential damage to both the material and the autoclave.
10. Wait for pressure and temperature to drop before opening the door
To avoid steam injuries and broken glassware, wait until the pressure has dropped to zero and the temperature has dropped to or below 121°C before opening the autoclave door after a cycle.
11. Handle overheated liquids with caution
If liquids in the autoclave reach a temperature above their boiling point without boiling, known as superheating, they can become unstable. Any movement or disturbance of the liquid can cause it to rapidly boil and spray violently. Take care when unloading the autoclave to avoid any accidents.
12. Do not autoclave sharp materials, hazardous chemicals, bleach-associated material, low molecular weight biotoxins, and pathogenic wastes
These materials should never be autoclaved as they can pose risks to both the operator and the autoclave.
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