Gravity displacement autoclaves and prevacuum sterilizers function differently primarily in how they remove air from the sterilization chamber and the efficiency of steam penetration into the load.
4 Key Differences Between Gravity Displacement Autoclaves and Prevacuum Sterilizers
1. Mechanism of Air Removal
Gravity displacement autoclaves rely on the natural buoyancy of steam to displace air.
Prevacuum sterilizers use a vacuum pump to remove air, enhancing steam penetration and sterilization efficiency.
2. Steam Introduction and Air Displacement
In gravity displacement autoclaves, steam is introduced at the top or sides of the sterilizing chamber.
Steam, being lighter than air, naturally forces air out through the bottom of the chamber via a drain vent.
3. Efficiency with Porous Loads
Gravity displacement autoclaves are commonly used for processing laboratory media, water, pharmaceutical products, regulated medical waste, and nonporous articles.
However, the penetration time into porous items is prolonged due to incomplete air elimination.
For instance, decontaminating 10 lbs of microbiological waste requires at least 45 minutes at 121°C because the remaining entrapped air in the load significantly retards steam permeation and heating efficiency.
Prevacuum sterilizers are equipped with a vacuum pump or ejector that ensures complete air removal from the sterilizing chamber and the load before steam is admitted.
This results in nearly instantaneous steam penetration even into porous loads, significantly reducing the sterilization time and improving the effectiveness of the process.
4. Testing and Maintenance
The Bowie-Dick test is used to ensure proper functioning of prevacuum sterilizers, checking for air leaks and adequate air removal.
This test involves placing a test pack in an empty chamber and running it at 134°C for 3.5 minutes.
If the sterilizer fails this test, it must be inspected and repaired before further use.
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