Knowledge What Cannot be Sterilized in an Autoclave? 5 Key Materials to Avoid
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Tech Team · Kintek Solution

Updated 2 months ago

What Cannot be Sterilized in an Autoclave? 5 Key Materials to Avoid

An autoclave is a trusted sterilizer that uses high heat and steam to kill microorganisms and achieve sterilization.

However, there are certain materials and substances that cannot be sterilized in an autoclave.

Here is a detailed list of what cannot be sterilized in an autoclave:

1. Acids

What Cannot be Sterilized in an Autoclave? 5 Key Materials to Avoid

Autoclaves cannot be used to sterilize acids.

Acids can cause corrosion or react with the high heat and pressure inside the autoclave.

2. Explosive Material

Autoclaves cannot be used to sterilize explosive materials.

The high heat and pressure can potentially cause an explosion.

3. Flammable Material

Autoclaves cannot be used to sterilize flammable materials.

The heat and steam can ignite these substances.

4. Chlorine Based Products

Autoclaves cannot be used to sterilize chlorine-based products or products that contain chlorine.

These substances can react with the heat and pressure inside the autoclave and release toxic gases.

5. Reactive, Corrosive, or Toxic Materials

Autoclaves should not be used to sterilize materials that are reactive, corrosive, or toxic.

The heat and pressure inside the autoclave can cause these materials to react, corrode, or release harmful gases.

6. Radioactive Material

Autoclaves cannot be used to sterilize radioactive materials.

The heat and pressure inside the autoclave do not eliminate radioactivity and can potentially spread it.

In addition to the above, there are certain limitations and precautions to consider when using an autoclave for sterilization:

  • Autoclaves cannot be used for heat-sensitive materials or sharp-edged instruments, especially high-grade carbon steel scissors and scalpel blade edges.

  • Some materials, such as fabric and linens, cannot be sterilized in an autoclave without being destroyed.

  • High heat can cause some plasticware to melt, and sharp equipment can become dull.

  • Certain compounds degrade during sterilization, and oily substances cannot be handled as they do not combine with water.

  • Certain high-protein solutions, such as urea, vaccinations, and serums, may need to be filtered without heat as they degrade in excessive heat.

It is important to follow precautions while using an autoclave:

  • Autoclaves should not be used for sterilizing waterproof materials, such as oil and grease, or dry materials, such as glove powder.

  • Materials should be loaded in a way that allows efficient steam penetration, without overfilling the chamber.

  • Wrapping objects in aluminum foil is not recommended as it may interfere with steam penetration. Articles should be wrapped in materials that allow steam penetration.

  • Materials should not touch the sides or top of the chamber.

  • Clean items and waste should be autoclaved separately.

  • Polyethylene trays should not be used as they may melt and cause damage to the autoclave.

In conclusion, an autoclave is a highly effective sterilizer, but there are certain materials and substances that cannot be sterilized in it due to their chemical properties or the potential for damage.

It is important to consider these limitations and precautions when using an autoclave for sterilization purposes.

Continue exploring, consult our experts

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