Knowledge Why 121°C is Used in Autoclaves: 4 Key Reasons for Effective Sterilization
Author avatar

Tech Team · Kintek Solution

Updated 2 months ago

Why 121°C is Used in Autoclaves: 4 Key Reasons for Effective Sterilization

Autoclaves are essential tools in various fields, from healthcare to laboratory research, for their ability to sterilize equipment and materials. One of the most common settings for these devices is 121°C. But why exactly is this temperature used? Here’s a detailed look at the reasons behind this choice.

1. Temperature and Pressure Combination

Why 121°C is Used in Autoclaves: 4 Key Reasons for Effective Sterilization

Autoclaves use pressurized steam to reach high temperatures. At 121°C and a pressure of 15 psi (103 kPa), the environment becomes lethal to most microorganisms. This temperature is lethal because it denatures proteins and disrupts cellular functions in bacteria, viruses, and spores, leading to their death.

2. Time Duration

Sterilization at 121°C typically takes around 15-20 minutes for a standard load. However, the duration can vary based on the size of the load and the materials being sterilized. For instance, after autoclaving biological waste, running the autoclave empty at 121°C for 30 minutes ensures proper sterilization of the chamber and baskets.

3. Validation and Effectiveness

To ensure the autoclave is working effectively, biological indicators containing spores of Geobacillus stearothermophilus are used. These spores are known for their resistance to heat and are used to test the autoclave's performance. If the spores do not survive the sterilization process, it indicates that the autoclave is functioning correctly.

4. Importance of Correct Temperature and Pressure

Maintaining the correct temperature and pressure is crucial for consistent and reliable sterilization. This is essential in preventing the spread of infections and ensuring the integrity of experiments and procedures. Operating autoclaves at the recommended temperatures and pressures ensures compliance with health and safety regulations and standards, which is particularly important in healthcare and laboratory settings.

In summary, 121°C is a critical temperature setting in autoclaves because it provides a reliable and effective means of sterilization, ensuring the destruction of microorganisms and maintaining the safety and integrity of sterilized materials.

Continue Exploring, Consult Our Experts

Unlock the Power of Sterilization with KINTEK Autoclaves!

Ensure the highest standards of safety and efficiency in your laboratory with KINTEK's advanced autoclaves, designed to maintain precise temperatures of 121°C for optimal sterilization. Our autoclaves are engineered to deliver consistent results, ensuring that every instrument and material is thoroughly decontaminated. Trust KINTEK for your sterilization needs and experience the peace of mind that comes with reliable, compliant, and effective equipment. Contact us today to learn more about how our autoclaves can enhance your lab's performance and safety protocols.

Related Products

Portable autoclave sterilization pressure

Portable autoclave sterilization pressure

Portable autoclave sterilization pressure is a device that uses pressure saturated steam to quickly and effectively sterilize items.

Horizontal Autoclave Steam Sterilizer

Horizontal Autoclave Steam Sterilizer

The horizontal autoclave steam sterilizer adopts the gravity displacement method to remove the cold air in the inner chamber, so that the inner steam and cold air content is less, and the sterilization is more reliable.

Desktop fast autoclave sterilizer 16L / 24L

Desktop fast autoclave sterilizer 16L / 24L

The desktop fast steam sterilizer is a compact and reliable device used for rapid sterilization of medical, pharmaceutical, and research items.

Desktop fast autoclave sterilizer 20L / 24L

Desktop fast autoclave sterilizer 20L / 24L

The desktop fast steam sterilizer is a compact and reliable device used for rapid sterilization of medical, pharmaceutical, and research items.

Portable autoclave sterilization pressure (Digital display automatic type)

Portable autoclave sterilization pressure (Digital display automatic type)

Portable autoclave sterilization pressure is a device that uses pressure saturated steam to quickly and effectively sterilize items.

Desktop fast autoclave sterilizer 35L / 50L / 90L

Desktop fast autoclave sterilizer 35L / 50L / 90L

The desktop fast steam sterilizer is a compact and reliable device used for rapid sterilization of medical, pharmaceutical, and research items. It efficiently sterilizes surgical instruments, glassware, medicines, and resistant materials, making it suitable for various applications.

Horizontal Autoclave Steam Sterilizer (Microcomputer)

Horizontal Autoclave Steam Sterilizer (Microcomputer)

The horizontal autoclave steam sterilizer adopts the method of gravity displacement to remove the cold air in the inner chamber, so that the content of steam cold air in the inner chamber is less, and the sterilization is more reliable.

Herbal Powder Sterilization Autoclave Machine For Chinese Medicine

Herbal Powder Sterilization Autoclave Machine For Chinese Medicine

The herbal powder sterilization autoclave machine for Chinese medicine uses saturated steam for effective sterilization. It utilizes the heat and penetrative properties of steam, achieves sterilization after heat preservation, and maintains a good drying effect with a dedicated drying system.

Electric heating blast drying oven

Electric heating blast drying oven

The desktop fast autoclave sterilizer is a compact and reliable device used for rapid sterilization of medical, pharmaceutical, and research items.

Vertical pressure steam sterilizer (special for laboratory department)

Vertical pressure steam sterilizer (special for laboratory department)

Vertical pressure steam sterilizer is a kind of sterilization equipment with automatic control, which is composed of heating system, microcomputer control system and overheating and overpressure protection system.

Pulse Vacuum Lifting Sterilizer

Pulse Vacuum Lifting Sterilizer

The pulse vacuum lifting sterilizer is a state-of-the-art equipment for efficient and precise sterilization. It uses pulsating vacuum technology, customizable cycles, and a user-friendly design for easy operation and safety.

Hydrogen Peroxide Space Sterilizer

Hydrogen Peroxide Space Sterilizer

A hydrogen peroxide space sterilizer is a device that uses vaporized hydrogen peroxide to decontaminate enclosed spaces. It kills microorganisms by damaging their cellular components and genetic material.

Pulsating vacuum desktop steam sterilizer

Pulsating vacuum desktop steam sterilizer

The pulsating vacuum desktop steam sterilizer is a compact and reliable device used for rapid sterilization of medical, pharmaceutical, and research items.


Leave Your Message