The four main types of autoclaves are:
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Pressure Cooker Type/ Laboratory Bench Autoclaves (N-type): These are essentially modified domestic pressure cookers, suitable for small-scale sterilization tasks such as those needed by tissue culture enthusiasts or hobbyists. They are simple in design and operation, making them an economical choice for basic sterilization needs.
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Gravity Displacement Type Autoclave: This is the most commonly used type in research laboratories. It operates by introducing steam into the chamber, which then displaces the air by gravity through a drain port. The air is replaced by steam, which is a more effective medium for heat transfer, ensuring uniform heating and efficient sterilization.
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Positive Pressure Displacement Type (B-type): These are more advanced autoclaves where steam is generated in a separate steam generator and then introduced into the autoclave chamber. This type ensures rapid steam generation and can handle a variety of sterilization cycles, including those that require post-sterilization vacuum drying to ensure complete drying of the load.
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Negative Pressure Displacement Type (S-type): This type is the most expensive and sophisticated. It includes a vacuum generator and a steam generator, which work together to achieve high levels of sterilization efficiency. The vacuum helps to remove air from the chamber more effectively than gravity displacement, allowing for better steam penetration and thus more thorough sterilization.
Each type of autoclave is designed to meet specific needs and requirements, ranging from basic small-scale applications to advanced, large-scale sterilization processes. The choice of autoclave depends on the specific requirements of the materials to be sterilized, the volume of materials, and the level of sterility required.
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