What is the FTIR used to measure? Identify Chemical Bonds & Molecular Structure

In short, FTIR is used to measure how a sample absorbs infrared light. This measurement is not just a simple reading; it provides a detailed "fingerprint" of the sample's molecular structure by identifying the specific chemical bonds present. This technique is a cornerstone of chemical analysis, used to characterize new materials and to identify or verify known substances.

The core purpose of FTIR is not just to measure light absorption, but to translate that absorption data into a detailed map of a molecule's functional groups, effectively revealing its chemical identity.

How FTIR Reveals a Molecule's Identity

To understand what FTIR measures, you must first understand how molecules interact with light. The process is a powerful way to probe the very structure of matter.

The Core Principle: Molecular Vibrations

Chemical bonds within a molecule are not rigid; they are constantly in motion, vibrating by stretching and bending. Each type of bond (like a carbon-hydrogen bond, C-H, or a carbon-oxygen double bond, C=O) vibrates at a unique, characteristic frequency.

Infrared Light as a Probe

Infrared (IR) light is a form of energy. When a beam of IR light passes through a sample, the bonds that vibrate at the same frequency as the light will absorb that energy. Bonds that vibrate at different frequencies will let the light pass through unaffected.

From Light Absorption to a Spectrum

The FTIR instrument measures precisely which frequencies of IR light are absorbed by the sample and to what extent. It then plots this information on a graph called an infrared spectrum. This spectrum shows sharp peaks at the frequencies where absorption occurred.

Decoding the "Fingerprint"

Because different chemical bonds absorb at different, predictable frequencies, this spectrum acts as a unique molecular fingerprint. By analyzing the position and intensity of the peaks, a chemist can determine which functional groups are present in the molecule.

What Can You Actually Identify?

The practical applications of FTIR are vast, stemming from its ability to provide a detailed structural snapshot of a sample.

Identifying Functional Groups

This is the primary output of an FTIR analysis. The spectrum clearly shows peaks corresponding to specific groups of atoms. For example, a broad peak around 3300 cm⁻¹ typically indicates an O-H bond (found in alcohols), while a sharp, strong peak near 1700 cm⁻¹ points to a C=O bond (found in ketones and acids).

Verifying Known Samples

If you have a substance that is supposed to be, for example, pure aspirin, you can run an FTIR scan and compare its spectrum to a reference spectrum of pure aspirin. If the spectra match perfectly, you can confirm the sample's identity and purity. Any extra peaks would indicate impurities.

Characterizing New Materials

In research and development, when scientists synthesize new molecules, they use FTIR to confirm that the reaction was successful. The spectrum can verify that the desired chemical bonds have formed and that bonds from the starting materials have disappeared.

Understanding the Limitations

While incredibly powerful, FTIR is not a universal solution and has important constraints that every analyst must understand.

Not All Bonds are Visible

For a bond to absorb infrared light, its vibration must cause a change in the molecule's dipole moment. Highly symmetrical bonds, such as the nitrogen-nitrogen bond in N₂ or the oxygen-oxygen bond in O₂, do not meet this requirement and are therefore "invisible" to FTIR.

Complexity of Mixtures

Analyzing a complex mixture of several different compounds can be challenging. The spectra of all the components will overlap, creating a complicated graph that can be very difficult to interpret accurately without advanced analytical techniques.

Interpretation Requires Expertise

While the instrument generates the data, interpreting an FTIR spectrum correctly requires knowledge and experience. Identifying peaks is one step, but understanding their context and what they imply about the overall molecular structure is a skill.

How to Apply FTIR to Your Goal

The way you use the data from an FTIR analysis depends entirely on your objective.

• If your primary focus is identifying an unknown substance: You should compare your sample's full spectrum to a spectral database, paying close attention to the unique pattern in the "fingerprint region" (typically below 1500 cm⁻¹).
• If your primary focus is verifying the purity of a sample: You should look for the presence of small, unexpected peaks in the spectrum that do not match the reference material, as these signify contaminants.
• If your primary focus is characterizing a new material: You should analyze the spectrum to confirm the presence of peaks for the expected functional groups and, just as importantly, the absence of peaks from your starting materials.

Ultimately, FTIR provides a direct and reliable method for translating a molecule's invisible vibrations into clear, actionable chemical insights.

Summary Table:

FTIR Application	Key Measurement	Primary Use Case
Identify Functional Groups	Absorption peaks at specific frequencies (e.g., C=O, O-H)	Determine the types of chemical bonds present
Verify Sample Identity/Purity	Comparison to a reference spectrum	Confirm a substance is what it claims to be
Characterize New Materials	Presence/absence of expected bond vibrations	Confirm successful synthesis in R&D

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