Knowledge Why KBr Pellets are Used to Prepare Solid Samples in FTIR? 5 Key Reasons
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Tech Team · Kintek Solution

Updated 2 months ago

Why KBr Pellets are Used to Prepare Solid Samples in FTIR? 5 Key Reasons

KBr pellets are used to prepare solid samples in FTIR because they provide a transparent medium for infrared light, allowing for accurate and sensitive measurements of the sample's molecular structure.

The pellets are made by mixing the sample with KBr in a specific ratio, typically 1:100, and then compressing the mixture into a thin, transparent disk.

This method ensures that the sample is sufficiently diluted to avoid scattering or complete absorption of the IR beam, which would result in noisy spectra.

5 Key Reasons Why KBr Pellets are Used in FTIR

Why KBr Pellets are Used to Prepare Solid Samples in FTIR? 5 Key Reasons

1. Transparency to Infrared Light

KBr is transparent to infrared light, which is essential for FTIR spectroscopy.

The goal of preparing a sample for FTIR is to create a thin, transparent pellet that allows the IR beam to pass through with minimal interference.

KBr, being IR transparent, serves as an ideal matrix for embedding the sample.

2. Sample Concentration

The concentration of the sample in the KBr pellet is kept low, typically between 0.2 to 1 percent.

This low concentration is necessary because the pellet is thicker than a liquid film, and according to Beer's Law, a lower concentration is required to prevent the IR beam from being completely absorbed or scattered.

Too high a concentration can lead to noisy spectra and unreliable data.

3. Sensitive Detection

FTIR is a very sensitive technique, capable of detecting small amounts of sample.

The use of KBr pellets allows for the incorporation of just a few milligrams of the sample, with the majority of the pellet being composed of KBr.

This setup ensures that the sample is within the detection range of the FTIR instrument while maintaining the transparency necessary for accurate readings.

4. Standardized Procedure

The preparation of KBr pellets for FTIR is a standardized procedure that ensures consistency in sample presentation.

The pellets are typically required to be within a specific diameter range (3 mm to 13 mm), which depends on the manufacturer of the FTIR equipment.

This standardization helps in obtaining reproducible and comparable spectra across different samples and experiments.

5. Enhanced Spectral Quality

Using KBr pellets enhances the quality of the IR spectra by allowing the radiations to pass through the sample without significant hindrance.

This results in sharp peaks, good intensity, and high resolution, which are crucial for accurate molecular structure analysis.

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