Knowledge What is the Sample KBr Ratio for FTIR? (4 Key Steps to Perfect Preparation)
Author avatar

Tech Team · Kintek Solution

Updated 2 months ago

What is the Sample KBr Ratio for FTIR? (4 Key Steps to Perfect Preparation)

The typical sample to KBr ratio for FTIR (Fourier transform infrared spectroscopy) is usually around 1% by weight.

This method involves preparing a KBr pellet containing the sample, which is then analyzed using infrared light.

The use of KBr as a diluent is practical because it is transparent to infrared light, allowing for accurate measurements without blocking the light path.

What is the Sample KBr Ratio for FTIR? (4 Key Steps to Perfect Preparation)

What is the Sample KBr Ratio for FTIR? (4 Key Steps to Perfect Preparation)

1. Background Measurement

Initially, a background measurement is performed on pure KBr or another diluent powder packed into the sample plate of the diffuse reflectance accessory.

This step ensures that the subsequent measurements are accurate by accounting for the inherent properties of the diluent.

2. Sample Dilution

The sample powder is then diluted to a concentration range of 0.1% to 10% in KBr powder.

This dilution is crucial as it prevents the sample from blocking the light path and ensures that the detector can accurately measure the light absorption.

3. Packing the Sample Plate

After dilution, the sample is packed into the sample plate.

This can be done by filling the plate with KBr powder and then adding the sample powder mixed with a small quantity of KBr.

The amount of sample used is minimal, typically around 50 to 100 ng, which is sufficient for analysis.

4. Importance of Proper Sample Preparation

A high sample to KBr ratio can lead to cloudy disks or poor quality spectra.

The ideal ratio is maintained to ensure that the sample does not interfere with the light transmission and that the peaks in the spectrum are sharp and well-defined.

The sample is usually ground with an excess of KBr and pressed into a pellet using a die.

The size of the die can vary, but a common size is 13 mm in internal diameter.

Proper grinding and pressing techniques are essential to avoid issues such as pellet cloudiness or poor spectral quality.

Most detectors have a linear response over a specific range.

For FTIR, the largest peak caused by the sample should ideally have an intensity of 2-5 percent T.

Higher intensities can lead to distorted readings, as the detector may not accurately measure peaks above its linear range.

Continue exploring, consult our experts

Discover the precision of KINTEK SOLUTION's FTIR accessories, where the ideal 1% sample to KBr ratio is seamlessly integrated for optimal performance.

Elevate your spectroscopy analysis with our high-quality KBr pellets and diffuse reflectance accessories, designed to ensure crystal-clear measurements and accurate spectral data.

Trust KINTEK SOLUTION for all your laboratory material needs—your success is our priority. Start improving your research with KINTEK SOLUTION today!

Related Products

Automatic Lab XRF & KBR Pellet Press 30T / 40T / 60T

Automatic Lab XRF & KBR Pellet Press 30T / 40T / 60T

Fast and easy xrf sample pellet preparation with KinTek Automatic Lab Pellet Press. Versatile and accurate results for X-ray fluorescence analysis.

kbr pellet press 2T

kbr pellet press 2T

Introducing the KINTEK KBR Press - a handheld laboratory hydraulic press designed for entry-level users.

lab infrared press mold

lab infrared press mold

Easily release samples from our lab infrared press mold for accurate testing. Ideal for battery, cement, ceramics, and other sample preparation research. Customizable sizes available.

Two-dimensional vibrating sieve

Two-dimensional vibrating sieve

KT-VT150 is a desktop sample processing instrument for both sieving and grinding. Grinding and sieving can be used both dry and wet. The vibration amplitude is 5mm and the vibration frequency is 3000-3600 times/min.

Liquid nitrogen cryogenic vibration ball mill

Liquid nitrogen cryogenic vibration ball mill

Kt-VBM100 is a laboratory desktop high-performance vibrating ball mill and sieving dual-purpose small and lightweight instrument. The vibrating platform with a vibration frequency of 36,000 times/min provides energy.

Electric Hydraulic Pellet Press for XRF & KBR 20T / 30T / 40T / 60T

Electric Hydraulic Pellet Press for XRF & KBR 20T / 30T / 40T / 60T

Efficiently prepare samples with the Electric Hydraulic Press. Compact and portable, it's perfect for labs and can work in a vacuum environment.

XRF Boric Acid lab Powder Pellet Pressing Mold

XRF Boric Acid lab Powder Pellet Pressing Mold

Get accurate results with our XRF Boric Acid lab Powder Pellet Pressing Mold. Perfect for preparing samples for X-ray fluorescence spectrometry. Custom sizes available.

Potassium Fluoride (KF) Sputtering Target / Powder / Wire / Block / Granule

Potassium Fluoride (KF) Sputtering Target / Powder / Wire / Block / Granule

Get top-quality Potassium Fluoride (KF) materials for your lab needs at great prices. Our tailored purities, shapes, and sizes suit your unique requirements. Find sputtering targets, coating materials, and more.

Hybrid Tissue Grinder

Hybrid Tissue Grinder

KT-MT20 is a versatile laboratory device used for rapid grinding or mixing of small samples, whether dry, wet, or frozen. It comes with two 50ml ball mill jars and various cell wall breaking adapters for biological applications such as DNA/RNA and protein extraction.

Hybrid High Energy Vibratory Ball Mill

Hybrid High Energy Vibratory Ball Mill

KT-BM400 is used for rapid grinding or mixing of dry, wet and frozen small amount of samples in the laboratory. It can be configured with two 50ml ball mill jars

Micro Tissue Grinder

Micro Tissue Grinder

KT-MT10 is a miniature ball mill with a compact structure design. The width and depth are only 15X21 cm, and the total weight is only 8 kg. It can be used with a minimum 0.2ml centrifuge tube or a maximum 15ml ball mill jar.


Leave Your Message