Preparing a KBr pellet for FTIR involves several crucial steps to ensure the pellet is transparent and suitable for infrared transmission. This process is essential for accurate and sensitive spectroscopic analysis.
4 Steps to Perfect KBr Pellets for FTIR
1. Sample and KBr Mixture
The first step is to mix the sample with KBr powder. The sample should be about 0.1 to 1.0% by weight of the total mixture. This means for every 200 to 250 mg of KBr, only 0.2 to 2.5 mg of sample is used. This high ratio ensures that the pellet remains transparent and suitable for infrared transmission.
2. Pulverization and Drying
After mixing, the KBr and sample mixture is finely pulverized to ensure a homogeneous distribution of the sample within the KBr matrix. The KBr powder itself should be pulverized to a maximum of 200 mesh. The KBr powder is then dried at approximately 110°C for two to three hours to remove any moisture. It's important to dry the KBr slowly to prevent oxidation, which can lead to discoloration.
3. Degassing and Pellet Formation
The dried KBr and sample mixture is placed into a pellet-forming die. The mixture is then subjected to a vacuum of several mm Hg to remove any remaining air or moisture. Applying a vacuum helps in creating stronger, more transparent pellets that do not scatter light. A pressing load of approximately 8 tons is then applied for several minutes to form the pellet.
4. Storage
After the pellet is formed, it should be stored in a desiccator to prevent moisture absorption, which could affect the quality of the FTIR spectra.
Continue exploring, consult our experts
Discover the Precision of KINTEK SOLUTIONs for Your FTIR Spectroscopy Needs! KINTEK SOLUTION offers all the essential tools and knowledge you need to effortlessly create high-quality KBr pellets for FTIR analysis. From precise ratios of KBr to sample, to expert tips on pulverization and pellet formation, our comprehensive solutions ensure your spectroscopic data is accurate and reliable. Elevate your research with KINTEK SOLUTION – where innovation meets precision! 🌟
