KBr pellets are used in FTIR primarily because they provide a practical method for introducing a controlled and appropriate amount of sample into the system.
They ensure that the sample does not block the infrared light and allows for accurate spectral analysis.
KBr is chosen due to its transparency to infrared light, making it an ideal medium for preparing samples for FTIR analysis.
Why are KBr pellets used in FTIR? (4 Key Reasons)
1. Transparency to Infrared Light
KBr is transparent in the infrared region, which is crucial for FTIR where the sample must not absorb or block the infrared light being used for analysis.
This transparency allows the infrared light to interact with the sample without significant attenuation.
It ensures that the spectral data obtained is reliable and not distorted by the medium containing the sample.
2. Sample Preparation and Concentration
The KBr pellet method involves mixing the sample with KBr in a ratio typically around 1:100 (sample to KBr).
This dilution ensures that the sample does not overwhelm the system and that the FTIR can accurately detect and analyze the spectral characteristics of the sample.
The mixture is then compressed using a hydraulic press to form a pellet.
This process creates a thin, transparent pellet that contains the sample in an IR-transparent medium, facilitating the transmission of infrared light through the sample.
3. Compatibility with FTIR Equipment
The size of the KBr pellets is standardized to fit the specifications of FTIR equipment, typically ranging from 3 mm to 13 mm in diameter.
This compatibility ensures that the pellets can be used effectively with various FTIR instruments, allowing for consistent and reproducible results.
4. Versatility and Ease of Use
The KBr pellet method is widely used because it is straightforward and versatile, applicable to a variety of sample types, including powders and solids.
The method does not require complex or specialized equipment beyond a standard hydraulic press, making it accessible for many laboratories.
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