Knowledge What are the 6 Key Steps Involved in Sample Preparation?
Author avatar

Tech Team · Kintek Solution

Updated 1 month ago

What are the 6 Key Steps Involved in Sample Preparation?

Sample preparation is a crucial process that varies depending on the specific application and analysis technique.

What are the 6 Key Steps Involved in Sample Preparation?

What are the 6 Key Steps Involved in Sample Preparation?

1. Sample Collection

This is the initial step where a representative sample is collected from the source.

It is important to ensure proper handling and storage of the sample to maintain its integrity.

2. Sample Enrichment

In some cases, the sample may need to undergo enrichment to increase the concentration of the target analyte.

This can involve techniques such as filtration, centrifugation, or extraction.

3. Sample Preparation

This step involves processing the sample to extract or isolate the analyte of interest.

For example, in nucleic acid analysis, this step may involve nucleic acid extraction techniques.

The goal is to obtain a pure and concentrated sample for further analysis.

4. Sample Quantification/QC

Once the sample has been prepared, it is important to quantify the amount of analyte present.

This can be done using various techniques such as spectrophotometry or fluorimetry.

Quality control measures are also performed to ensure the accuracy and reliability of the sample.

5. Library Preparation & Amplification

In genomic analysis, this step involves the construction of libraries containing the DNA or RNA fragments of interest.

These libraries are then amplified to generate sufficient material for downstream analysis.

6. Target Enrichment

In some cases, it may be necessary to enrich specific regions or targets within the sample.

This can be done using techniques such as PCR or hybridization capture to selectively amplify or capture the desired targets.

It is important to follow proper safety protocols during sample preparation to ensure the safety of the operator and prevent contamination.

Additionally, the choice of milling and grinding methods, as well as the selection of appropriate equipment, should be based on the sample type and desired particle size.

Considering factors such as sample quantity, specific characteristics, and budget can help optimize the efficiency and effectiveness of the sample preparation process.

Continue exploring, consult our experts

Looking for high-quality laboratory equipment for sample preparation?

Look no further than KINTEK!

Our wide range of products ensures accurate and reproducible results for your analysis.

From sample collection to target enrichment, we have the tools you need to eliminate variability, increase sensitivity, and achieve accurate representations of your samples.

Trust KINTEK for all your sample preparation needs.

Contact us today!

Related Products

Vibration Mill

Vibration Mill

Vibration Mill for Efficient Sample Preparation, Suitable for Crushing and Grinding a Variety of Materials with Analytical Precision. Supports Dry / Wet / Cryogenic Grinding and Vacuum/Inert Gas Protection.

automatic heated lab pellet press 25T / 30T / 50T

automatic heated lab pellet press 25T / 30T / 50T

Efficiently prepare your samples with our Automatic Heated Lab Press. With a pressure range up to 50T and precise control, it's perfect for various industries.

Automatic Lab Pellet Press Machine 20T / 30T / 40T / 60T / 100T

Automatic Lab Pellet Press Machine 20T / 30T / 40T / 60T / 100T

Experience efficient sample preparation with our Automatic Lab Press Machine. Ideal for material research, pharmacy, ceramics, and more. Features a compact size and hydraulic press functionality with heating plates. Available in various sizes.

Hydraulic Heated Lab Pellet Press 24T / 30T / 60T

Hydraulic Heated Lab Pellet Press 24T / 30T / 60T

Looking for a reliable Hydraulic Heated Lab Press? Our 24T / 40T model is perfect for material research labs, pharmacy, ceramics, and more. With a small footprint and the ability to work inside a vacuum glove box, it's the efficient and versatile solution for your sample preparation needs.

Automatic Lab Cold Isostatic Press (CIP) 300MPA-400MPA-500MPA

Automatic Lab Cold Isostatic Press (CIP) 300MPA-400MPA-500MPA

Efficiently prepare samples with our Automatic Lab Cold Isostatic Press. Widely used in material research, pharmacy, and electronic industries. Provides greater flexibility and control compared to electric CIPs.

Lab pellet press machine for glove box

Lab pellet press machine for glove box

Controlled environment lab press machine for glove box. Specialized equipment for material pressing and shaping with high precision digital pressure gauge.

Hybrid Tissue Grinder

Hybrid Tissue Grinder

KT-MT20 is a versatile laboratory device used for rapid grinding or mixing of small samples, whether dry, wet, or frozen. It comes with two 50ml ball mill jars and various cell wall breaking adapters for biological applications such as DNA/RNA and protein extraction.

Manual Cold Isostatic Pellet Press (CIP) 12T / 20T / 40T / 60T

Manual Cold Isostatic Pellet Press (CIP) 12T / 20T / 40T / 60T

Lab Manual Isostatic Press is a high-efficient equipment for sample preparation widely used in material research, pharmacy, ceramics, and electronic industries. It allows for precision control of the pressing process and can work in a vacuum environment.

Electrode polishing material

Electrode polishing material

Looking for a way to polish your electrodes for electrochemical experiments? Our polishing materials are here to help! Follow our easy instructions for best results.

Manual Lab Pellet Press For Vacuum Box

Manual Lab Pellet Press For Vacuum Box

The lab press for vacuum box is a specialized piece of equipment designed for laboratory use. Its main purpose is to press pills and powders according to specific requirements.

Automatic Lab XRF & KBR Pellet Press 30T / 40T / 60T

Automatic Lab XRF & KBR Pellet Press 30T / 40T / 60T

Fast and easy xrf sample pellet preparation with KinTek Automatic Lab Pellet Press. Versatile and accurate results for X-ray fluorescence analysis.

Micro Tissue Grinder

Micro Tissue Grinder

KT-MT10 is a miniature ball mill with a compact structure design. The width and depth are only 15X21 cm, and the total weight is only 8 kg. It can be used with a minimum 0.2ml centrifuge tube or a maximum 15ml ball mill jar.

kbr pellet press 2T

kbr pellet press 2T

Introducing the KINTEK KBR Press - a handheld laboratory hydraulic press designed for entry-level users.

Electric Hydraulic Pellet Press for XRF & KBR 20T / 30T / 40T / 60T

Electric Hydraulic Pellet Press for XRF & KBR 20T / 30T / 40T / 60T

Efficiently prepare samples with the Electric Hydraulic Press. Compact and portable, it's perfect for labs and can work in a vacuum environment.

Two-dimensional vibrating sieve

Two-dimensional vibrating sieve

KT-VT150 is a desktop sample processing instrument for both sieving and grinding. Grinding and sieving can be used both dry and wet. The vibration amplitude is 5mm and the vibration frequency is 3000-3600 times/min.

High Throughput Tissue Grinder

High Throughput Tissue Grinder

KT-MT is a high-quality, small, and versatile tissue grinder used for crushing, grinding, mixing, and cell wall breaking in various fields, including food, medical, and environmental protection. It is equipped with 24 or 48 2ml adapters and ball grinding tanks and is widely employed for DNA, RNA, and protein extraction.


Leave Your Message