KBr pellets are a crucial component in FTIR (Fourier Transform Infrared Spectroscopy). They provide a practical and efficient way to introduce a controlled amount of sample into the system while ensuring transparency to infrared light. This transparency is essential for accurate and reliable spectral analysis.
Why is KBr Pellet Used in FTIR? 4 Key Reasons Explained
1. Transparency to Infrared Light
KBr is transparent to infrared light, which is vital for FTIR analysis. When a sample is mixed with KBr and compressed into a pellet, the resulting pellet allows infrared light to pass through. This enables the detection of absorption bands that correspond to the molecular structure of the sample.
2. Controlled Sample Amount
Using KBr pellets allows for precise control over the amount of sample used in the analysis. Typically, only about 1% of the pellet by weight is the actual sample, with the rest being KBr. This low sample requirement minimizes the amount of sample needed and prevents overloading the system.
3. Practicality and Versatility
The KBr pellet method is straightforward and widely applicable. It involves mixing the sample with KBr, usually in a 100:1 ratio, and then compressing this mixture under high pressure to form a transparent pellet. This method is particularly useful for samples that are not compatible with other sampling techniques or when higher sensitivity is required.
4. Enhanced Signal-to-Noise Ratio
Compared to other methods like Attenuated Total Reflectance (ATR), KBr pellets often provide a higher signal-to-noise ratio. This improvement is crucial for obtaining clear and reliable spectra, especially for complex samples or those with weak spectral features.
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