Knowledge Is KBr Used in IR Spectroscopy? 5 Key Points to Know
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Tech Team · Kintek Solution

Updated 2 months ago

Is KBr Used in IR Spectroscopy? 5 Key Points to Know

KBr is indeed used in IR spectroscopy, primarily for sample preparation to ensure accurate and high-resolution spectra.

KBr is chosen because it is transparent to infrared radiation, allowing the radiation to pass through effectively when mixed with the sample.

This transparency is crucial for obtaining clear and detailed spectra.

1. Sample Preparation with KBr

Is KBr Used in IR Spectroscopy? 5 Key Points to Know

In IR spectroscopy, samples are often mixed with KBr to form pellets.

These pellets are then analyzed to obtain the IR spectrum of the sample.

The process typically involves diluting the sample in KBr powder, usually at concentrations ranging from 0.1% to 10% by weight.

This mixture is then packed into a sample plate for measurement.

The use of KBr ensures that the sample is transparent to the IR radiation, allowing for the detection of sharp peaks and good intensity in the spectrum.

2. Background Measurement and Analysis

Before the sample is analyzed, a background measurement is performed on the KBr or other diluent powder.

This step is crucial as it helps in establishing a baseline for the subsequent sample measurements.

The sample, mixed with KBr, is then placed in the sample plate, and its infrared spectrum is measured.

This method allows for the analysis of very small sample volumes, as little as 50 to 100 ng.

3. FTIR Analysis and KBr Pellets

In Fourier Transform Infrared (FTIR) spectroscopy, KBr pellets are particularly useful.

FTIR involves comparing the light passing through the system with and without the sample.

Using KBr pellets helps in ensuring that the sample does not block the path of the light, which could otherwise lead to unreliable results.

Typically, the KBr pellet contains just 1% sample by weight, ensuring minimal obstruction of the light path.

4. Kubelka-Munk Transformation

When using diffuse reflectance accessories, the light undergoes repeated transmission through the sample, which can emphasize low absorption bands.

To compare these results with transmission spectra, a Kubelka-Munk transformation is applied to the diffuse reflectance spectrum.

This transformation allows for quantitative analysis and comparison with traditional transmission spectra.

5. Summary

In summary, KBr is extensively used in IR spectroscopy for sample preparation due to its transparency to infrared radiation.

This property allows for the creation of pellets that facilitate accurate and high-resolution spectral analysis, crucial for identifying chemical bonds and their vibrations in the sample.

Continue Exploring, Consult Our Experts

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