When using FTIR, several precautions must be taken to ensure safety and accuracy in the analysis. These precautions include proper handling and preparation of samples, safety measures during the use of furnaces, and maintenance of equipment.
Sample Preparation:
- Cleaning Equipment: Before preparing the sample, ensure that all equipment such as the die set, pestle, and mortar are thoroughly cleaned with a solvent and deionized (DI) water, then dried in a warm oven. This prevents contamination of the sample.
- Sample Quantity: The amount of sample used should be appropriate for the size of the pellet. For a ½ inch diameter sample, use approximately 1-2 mg of powder. Adjust the quantity based on the pellet size or follow the manufacturer's instructions.
Safety Measures During Furnace Use:
- Temperature Control: Heat materials to the lowest possible temperature to minimize the risk of burns and furnace failure.
- Ventilation: Operate furnaces in a well-ventilated area or in a fume hood to ensure proper ventilation of any potentially hazardous fumes.
- Training and Compliance: Furnace operators should undergo specific safety training and thoroughly read the instrument’s manual. They should also consult with the manufacturer and their principal investigator (PI) to ensure that experiments are suitable for the unit.
- Personal Protective Equipment (PPE): Always wear appropriate PPE such as long pants, closed-toe shoes, a lab coat, safety glasses, and thermal gloves when working with furnaces.
Equipment Maintenance:
- Cleaning KBr Plates: After use, clean the KBr plates thoroughly to prevent contamination of future samples. This involves wiping the windows with a tissue, washing several times with solvent and ethanol, and polishing the surface with a polishing kit.
- Observation of Furnace Operation: A lab furnace should always be under observation when in operation to detect any faults such as smoke or strong odors that could indicate a fire.
General Operational Precautions:
- Avoid Overheating: Do not exceed the maximum temperature of the furnace or use containers outside their recommended temperature range.
- Electrical Safety: Cut off the power supply when loading and taking samples to prevent electric shock.
- Handling Samples: Wear gloves when loading and taking samples to prevent scalding, and ensure the sample is placed in the middle of the furnace and arranged neatly.
By adhering to these precautions, the risk of accidents and errors in FTIR analysis can be significantly reduced, ensuring both safety and accuracy in laboratory operations.
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